Luis Suarez returned from injury to score twice as Uruguay beat England 2-1 to breathe new life into their FIFA World Cup campaign.
Uruguay were shocked by a 3-1 loss to Costa Rica in their opening Group D fixture last Saturday, but Suarez - who was absent for that game - proved the difference in Sao Paulo on this occasion.
The fitness of the Liverpool star dominated the pre-match headlines but, as expected, he was included from the start just four weeks after undergoing minor knee surgery.
And he marked his first appearance of this World Cup with a double, nodding in a superb Edinson Cavani cross six minutes before half-time to give Uruguay the lead.
Wayne Rooney missed two glorious chances for England in the first half, first heading into the bar from one yard while scores were level before shooting straight at Uruguay goalkeeper Fernando Muslera early in the second period.
But he made amends as he tapped in Glen Johnson's smart ball across the face of goal with 15 minutes left to equalise and set up a grandstand finish.
Suarez had the final say, though, pouncing on Muslera's long ball - which flicked the head of England captain Steven Gerrard - and firing past Joe Hart with just five minutes left to all but end England's chances of reaching the last 16.
Uruguay will now head into Tuesday's clash against Italy in buoyant mood, while England, beaten in both Group D matches, need a minor miracle to qualify.
Uruguay coach Oscar Tabarez threatened to ring the changes after the Costa Rica match and he was true to his word, making five alterations, as captain Diego Lugano (knee) and defender Maxi Pereira (suspended) were not the only casualties.
In stark contrast, England were unchanged, and Roy Hodgson's faith was nearly rewarded when Rooney - the target of much criticism following their 2-1 defeat to Italy - curled a 20-yard free-kick just wide with Muslera rooted to the spot.
Phil Jagielka's mishit clearance allowed Cristian Rodriguez in at the other end, but his fiercely hit left-foot effort sailed inches over the crossbar in the 15th minute.
Diego Godin was then perhaps lucky to stay on the pitch, as the defender, given a yellow card for a handball that led to Rooney's early free-kick, caught Daniel Sturridge by the throat with his forearm, but avoided further punishment.
England should have taken the lead in the 31st minute but Rooney, who rose above Martin Caceres, inexplicably headed into the bar from one yard from a Gerrard free-kick, and they were soon made to pay for their profligacy.
Nicolas Lodeiro instigated the opener, beating Gerrard in midfield, and he fed Cavani, who, from the left, produced an inch-perfect cross that looped over Jagielka and onto Suarez's head, with the striker nodding across Hart and in from eight yards.
Sturridge nearly produced a quick response for England but they had to weather a storm after the break as Uruguay ramped up the pressure.
Suarez caught Hart napping as he shot from a corner, with the goalkeeper having to scramble back to keep it out, and he also fired wide after England were caught out at the back.
Cavani then wasted a fine chance to double Uruguay's lead, shooting wide when one-on-one with Hart before Rooney could only shoot at Muslera when played in at the other end.
Rooney finally opened his World Cup account in the 75th minute as Johnson, who cut in from the right and beat Alvaro Gonzalez, saw the Manchester United man steal in front of Caceres and shoot past Muslera.
England's celebrations were cut short, though, as Muslera's hopeful punt forward saw Suarez pounce on Gerrard's inadvertent flick-on, the striker keeping his cool to slam the winner past Hart.
Aussies Abroad: Sainsbury denies former club Inter in first UEFA Champions League start
Two Caltex Socceroos defenders were involved in key UEFA Champions League matches on Wednesday morning (AEDT). Milos Degenek’s Red Star Belgrade took on the might of Paris Saint Germain, featuring Neymar and Kylian Mbappe, while Trent Sainsbury went to former club Inter Milan
Aussies Abroad: Nabbout, Urawa Red Diamonds win Emperors Cup final
Andrew Nabbout emerged an Emperors Cup Final winner with Urawa Red Diamonds after the J-League outfit defeated Vegalta Sendai 1-0 yesterday evening. Nabbout was an unused substitute as the Reds clinched the trophy thanks to an early strike from Tomoya Ugajin. It is Urawa’s
'Amazing reward': Cahill to feature in FIFA World Football Museum
A pair of Tim Cahill’s boots will join items like the FIFA World Cup trophy, match balls from some of football’s biggest occasions and rare match-worn shirts in FIFA’s World Football Museum in Switzerland. The Caltex Socceroos legend revealed on Instagram on Sunday morning (AE
Boyle given 'all clear' following 'nasty collision'
Caltex Socceroos attacker Martin Boyle has been given the “all clear” following a head knock in Hibernian’s Scottish Premiership win at Hamilton Academical on Sunday morning (AEDT). Boyle needed lengthy treatment before half time after clashing with Hamilton’s Gary Woods and s